EIF4E (phospho S209), Rabbit anti-Human, Mouse, Rat, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of EIF4E.

Supplier:  Abnova Corporation PAB25828

Catalog No. 89-127-457

Description

All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24kDa polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50kDa polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220kDa polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM

Sequence: S-G-Sp-T-T

Specifications

• Antigen: EIF4E
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
• Gene: EIF4E
• Gene Alias: CBP/EIF4E1/EIF4EL1/EIF4F/MGC111573
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1977
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

• Applications: Immunohistochemistry (PFA fixed), Western Blot
• Concentration: 1 mg/mL
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of EIF4E.
• Formulation: In PBS (without mg²⁺ and Ca²⁺), 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene Accession No.: P06730
• Gene Symbols: EIF4E
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S209 of human EIF4E.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Liquid

For Research Use Only