MilliporeSigma™ Rabbit anti-GRASP55, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABS1520

Catalog No. ABS1520MI

Description

Specifically detects GRASP55 in Hamster, Human, Mouse samples, and it is validated for Immunocytochemistry, Western Blotting

Golgi reassembly stacking protein of 55kDa (also called Golgi reassembly-stacking protein of 55kDa, GRASP55, or Grs2) is encoded by the gene designated as Gorasp2 in rat & mouse (GORASP2/GRS2/GOLPH6 in human). Golgi stack formation involves two homologous peripheral Golgi proteins, GRASP55 and GRASP65, localized to the medial-trans and cis cisternae, respectively. GRASP55 and GRASP65 stack mammalian Golgi cisternae via a common mechanism of phosphorylation-regulated oligomerization. GRASP55 stacks Golgi membranes by forming oligomers through its N-terminal GRASP domain. This process is regulated by phosphorylation within the C-terminal serine/proline-rich domain. Expression of nonphosphorylatable GRASP55 mutants enhances Golgi stacking in interphase cells and inhibits Golgi disassembly during mitosis. Knockdown of either GRASP55 or GRASP65 by siRNA reduces the number of cisternae per Golgi stack, while simultaneous knockdown of both GRASP proteins leads to disassembly of the entire stack. (PMID 20083603). Anti-GRASP55, is a rabbit polyclonal antiserum raised against a recombinant immunogen encompassing region within the C-terminal half of rat GRASP55.

Specifications

• Antigen: GRASP55
• Classification: Polyclonal
• Gene Accession No.: Q9R064
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Hamster, Human, Mouse
• Form: Serum

• Applications: Immunocytochemistry, Western Blot
• Formulation: Rabbit polyclonal serum with 0.05% sodium azide.
• Gene Symbols: Gorasp2
• Immunogen: Recombinant protein corresponding to the C-terminal of rat GRASP55.
• Quantity: 100 μL
• Research Discipline: Signaling
• Gene ID (Entrez): AF110267
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.

For Research Use Only