MilliporeSigma™ Rabbit anti-IRF7, Polyclonal,

Catalog No.	Quantity
ABF130MI	100 μL

Catalog No. ABF130MI Supplier MilliporeSigma™ Supplier No. ABF130

Description

Rabbit Polyclonal Antibody

Specifically detects IRF7 in Mouse samples, and it is validated for Chromatin Immunoprecipitation, Western Blotting

Mouse Interferon regulatory factor 7 or IRF-7 (UniProt P70434) is encoded by the murine irf7 gene (Entrez Gene ID 54123). IFN-γ-induced gene activations are mediated by both signal transducers and activators of transcription (STATs) and interferon regulatory factors (IRFs). STAT1 dimer, for example, stimulates immediate transcription by binding IFN-γ-activated site (GAS) sequence in the promoter region of target genes. While a delayed, secondary transcription activation is observed among IFN-γ target genes containing an IFN regulatory factors (IRF) binding site within the interferon-stimulated response element (ISRE) sequence. Irf7 transcription is shown to be activated by STAT1 and IRF9 in response to type II interferon (IFN) IFN-γ stimulation in MEF cultures. The expressed IRF7 protein then further cooperates with STAT1 and IRF1 to stimulate the expression of a subset of IFN-γ-induced STAT1 target genes (PMID 22252317). Anti-IRF7 is a rabbit polyclonal antiserum raised against recombinant GST fusion protein (PMID 22252317) encompassing murine IRF7 C-terminal half sequence. This antiserum specifically detects INF-gamma-induced IRF7 expression and chromatin association in Wt, but not Irf7-/- MEF by Western blotting and ChIP application, respectively (PMID 22252317). Murine IRF7 has a calculated M.W. of 51.2kDa, but migrates with an apparent M.W. of ∽60kDa by SDS-PAGE.

Specifications

• Antigen: IRF7
• Applications: ChIP Assay, Western Blot
• Classification: Polyclonal
• Formulation: Rabbit polyclonal serum with 0.05% sodium azide.
• Gene Accession No.: P70434
• Gene Symbols: Irf7
• Host Species: Rabbit
• Immunogen: GST-tagged recombinant protein corresponding to the C-terminal of mouse IRF7.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Inflammation and Immunology
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_016850
• Target Species: Mouse
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Form: Serum

For Research Use Only