MilliporeSigma™ Rabbit anti-Neph1, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABS1511

Catalog No. ABS1511MI

Description

Specifically detects Neph1 in Human, Mouse, Rat samples, and it is validated for Electron Microscopy, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blotting

Kin of IRRE-like protein 1 (UniProt Q80W68; also known as Nephrin-like protein 1, Kin of IRRE like 1, Kin of irregular chiasm-like protein 1) is encoded by the Kirrel (also known as 6720469N11Rik, Kirrel1, Neph1) gene (Gene ID 170643) in murine species. Podocytes are specialized epithelial cells that are critical components of the glomerular filtration barrier. Glomerular injury, such as that caused by renal ischemia, is often characterized by the effacement of podocytes, loss of slit diaphragms, and proteinuria. The podocyte proteins Neph1 and nephrin are critical for maintaining the structural integrity of slit diaphragm and therefore the filtration function of the glomerulus by organizing a signaling complex at the podocyte cell membrane. Cytoplasmic domain of Neph1 plays a key role in actin cytoskeleton remodeling events that are directly associated with Fyn-mediated Neph1 pTyr637/638 phosphorylation that leads to the recruitment of adapter proteins and signaling molecules, such as Grb2, Csk tyrosine kinase, and ZO-1. Phosphorylation-induced association between Neph1 and ZO-1 plays a key role in defining the tight junction formation in podocytes.

Specifications

• Antigen: Neph1
• Classification: Polyclonal
• Gene Accession No.: Q80W68
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat

• Applications: Immunoassay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
• Formulation: Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, 0.02% Sodium Azide and 30% glycerol.
• Gene Symbols: Kirrel; Kirrel1; Neph1
• Immunogen: His-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Neph1.
• Quantity: 100 μL
• Research Discipline: Signaling
• Gene ID (Entrez): NP_001164456
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

For Research Use Only