NFKBIA (phospho S32/S36), Rabbit anti-Human, Mouse, Rat, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of NFKBIA.

Supplier:  Abnova Corporation PAB12626

Catalog No. 89-115-961

Description

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM

Sequence: DSGLDSM

Specifications

• Antigen: NFKBIA
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of NFKBIA.
• Formulation: In TBS, pH 7.2 (BSA, 10% Proclin300)
• Gene Alias: IKBA/MAD-3/NFKBI
• Host Species: Rabbit
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes ∼42 KDa of human NFKBIA at dual phosphorylation sites of Ser32/Ser36. It does not recognize non-phosphorylated peptides.
• Content And Storage: Store at 4°C. For long term storage store at -20°C or lower.
  Aliquot to avoid repeated freezing and thawing.

• Applications: ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Dilution: Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user.
• Gene: NFKBIA
• Gene Symbols: NFKBIA
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S32/S36 of human NFKBIA.
• Regulatory Status: RUO
• Gene ID (Entrez): 4792
• Target Species: Human, Mouse, Rat
• Form: Liquid

For Research Use Only