MilliporeSigma™ Rabbit anti-phospho-Neph1 (Tyr637/638), Polyclonal,

Catalog No.	Quantity
ABS1509MI	100 μL

Catalog No. ABS1509MI Supplier MilliporeSigma™ Supplier No. ABS1509

Description

Rabbit Polyclonal Antibody

Specifically detects phospho-Neph1 (Tyr637/638) in Human samples, and it is validated for Western Blotting

Kin of IRRE-like protein 1 (UniProt Q80W68; also known as Nephrin-like protein 1, Kin of IRRE like 1, Kin of irregular chiasm-like protein 1) is encoded by the Kirrel (also known as 6720469N11Rik, Kirrel1, Neph1) gene (Gene ID 170643) in murine species. Podocytes are specialized epithelial cells that are critical components of the glomerular filtration barrier. Glomerular injury, such as that caused by renal ischemia, is often characterized by the effacement of podocytes, loss of slit diaphragms, and proteinuria. The podocyte proteins Neph1 and nephrin are critical for maintaining the structural integrity of slit diaphragm and therefore the filtration function of the glomerulus by organizing a signaling complex at the podocyte cell membrane. The cytoplasmic domain of Neph1 plays a key role in actin cytoskeleton remodeling events that are directly associated with Fyn-mediated Neph1 pTyr637/638 phosphorylation that leads to the recruitment of adapter proteins and signaling molecules, such as Grb2, Csk tyrosine kinase, and ZO-1. Phosphorylation-induced association between Neph1 and ZO-1 plays a key role in defining the tight junction formation in podocytes.

Specifications

• Antigen: phospho-Neph1 (Tyr637/638)
• Applications: Western Blot
• Classification: Polyclonal
• Formulation: Purified rabbit polyclonal antibody in buffer containing PBS/glycine/5% BSA, 0.02% Sodium Azide and 30% glycerol.
• Gene Accession No.: Q80W68
• Gene Symbols: Kirrel; Kirrel1; Neph1
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to the cytoplasmic domain of mouse phospho-Neph1 (Tyr637/638).
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001164456
• Target Species: Human
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

For Research Use Only