MilliporeSigma™ Rabbit anti-phospho- SMAD2 (Ser465/467), Polyclonal,

Catalog No.	Quantity
AB3849I	100 μg

Catalog No. AB3849I Supplier MilliporeSigma™ Supplier No. AB3849I

Description

Rabbit Polyclonal Antibody

Specifically detects phospho- SMAD2 (Ser465/467) in Human samples, and it is validated for Western Blotting

Mothers against decapentaplegic homolog 2 (UniProt Q15796; also known as hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, Smad2) is encoded by the SMAD2 (also known as MADH2, MADR2) gene (Gene ID 4087) in human. Smad2 is a receptor-regulated intracellular signal transducer and transcriptional modulator activated by transforming growth factor TGF-beta and activin type 1 receptor kinases. In response to TGF-beta signal, smad2 is recruited to the activated TGF-beta receptor through its interaction with the SMAD anchor for receptor activation (SARA) protein, where it is phosphorylated by the TGF-beta receptor. The phosphorylation results in its dissociation from SARA and association with another family member smad4. The smad2-smad4 complex then translocates into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Smad2 can also be phosphorylated by activin type 1 receptor kinase, and thereby transduce activin-dependent signaling events.

Specifications

• Antigen: phospho- SMAD2 (Ser465/467)
• Applications: Western Blot
• Classification: Polyclonal
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: Q15796
• Gene Symbols: SMAD2; MADH2; MADR2
• Host Species: Rabbit
• Immunogen: KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
• Purification Method: Affinity Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_005892
• Target Species: Human
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.

For Research Use Only