PPP1R1B (phospho T75), Rabbit anti-Human, Mouse, Rat, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of PPP1R1B.

Supplier:  Abnova Corporation PAB25435

Catalog No. 89-127-052

Description

Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders. One well-studied target for the actions of dopamine is DARPP32. In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons (Ouimet and Greengard, 1990 [PubMed 2191086]) that also express dopamine D1 receptors (Walaas and Greengard, 1984 [PubMed 6319627]). The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions (Halpain et al., 1990 [PubMed 2153935]). Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 (Walaas and Greengard, 1984 [PubMed 6319627]); phosphorylated DARPP32 is a potent protein phosphatase-1 (see MIM 176875) inhibitor (Hemmings et al., 1984 [PubMed 6087160]). NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32 (Halpain et al., 1990 [PubMed 2153935]).[supplied by OMIM

Sequence: A-Y-Tp-P-P

Specifications

• Antigen: PPP1R1B
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of PPP1R1B.
• Formulation: In PBS (without Mg²⁺ and Ca²⁺), 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene Alias: DARPP-32/DARPP32/FLJ20940
• Host Species: Rabbit
• Purification Method: Affinity purification
• Regulatory Status: RUO
• Gene ID (Entrez): 84152
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

• Applications: Immunofluorescence
• Conjugate: Unconjugated
• Dilution: Immunofluorescence (1:100-1:200) The optimal working dilution should be determined by the end user.
• Gene: PPP1R1B
• Gene Symbols: PPP1R1B
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T75 of human PPP1R1B.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Liquid

For Research Use Only