MilliporeSigma™ Rabbit anti-RAD18, Unpurified, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABE1377

Catalog No. ABE1377MI

Description

Specifically detects RAD18 in Human, Mouse samples, and it is validated for Immunocytochemistry, Immunohistochemistry, Western Blotting

Human Rad18 (hRad18) is a homologue of the S. pombe Rad18. In yeast, Rad6 and Rad18 play a crucial role in post−replication repair functions in gap−filling of daughter strands on replication of damaged DNA. Furthermore, Rad6−Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring−finger motif. It has been suggested that the Rad18−Rad6 complex binds to the damaged sites by the single−stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by the ubiquitin−conjugating enzyme activity of Rad6. The human Rad18 maps on chromosome 3p24−25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56kDa. The human Rad18 is ubiquitously expressed in various human tissues.

Specifications

• Antigen: RAD18
• Classification: Polyclonal
• Gene Accession No.: Q9QXK2
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Form: Serum

• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Formulation: Rabbit polyclonal serum with 0.02% sodium azide.
• Gene Symbols: Rad18; Rad18sc
• Immunogen: GST-tagged recombinant mouse RAD18 C-terminal fragment.
• Quantity: 100 μL
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_067360
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.

For Research Use Only