RAF1 (phospho Y341), Rabbit anti-Human, Mouse, Polyclonal Antibody, Abnova™

Catalog No.	Quantity
89-130-702	100 μL

Catalog No. 89-130-702 Supplier Abnova Corporation Supplier No. PAB29265

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RAF1.

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq

Specifications

• Antigen: RAF1
• Applications: Immunohistochemistry (PFA fixed), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RAF1.
• Dilution: Immunohistochemistry (1:50∼1:100) Western Blot (1:500∼1:1000) The optimal working dilution should be determined by the end user.
• Formulation: In PBS (without Mg²⁺ and Ca²⁺), 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene: RAF1
• Gene Alias: CRAF/NS5/Raf-1/c-Raf
• Gene Symbols: RAF1
• Host Species: Rabbit
• Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y341 of human RAF1.
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 5894
• Test Specificity: RAF1 (phospho Y341) polyclonal antibody detects endogenous levels of human RAF1 only when phosphorylated at tyrosine 341.
• Target Species: Human, Mouse
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid

For Research Use Only