MilliporeSigma™ Rabbit anti-RBPJ, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABE384

Catalog No. ABE384MI

Description

Specifically detects RBPJ in Human, Mouse samples, and it is validated for ChIP, ChIP-seq, Western Blotting

Recombining binding protein suppressor of hairless (UniProt Q06330; also known as CBF-1, csI, H-2K binding factor-2, Immunoglobulin kappa J region recombination signal binding protein 1, J kappa-recombination signal-binding protein, RBP-J, RBP-JK, RBP-J kappa, Renal carcinoma antigen NY-REN-30, Suppressor of hairless homolog) is encoded by the RBPJ (also known as AOS3, CBF1, IGKJRB, IGKJRB1, KBF2, RBPJK, RBPSUH, SUH) gene (Gene ID 3516) in human. RBPJ/RBP-JK is a DNA-binding protein that functions as a transcriptional switch and plays an important role in regulating Notch signaling. RBPJ associates with several transcriptional co-repressors in the absence of Notch intracellular domain (NICD). Upon activation by gamma-secretase cleavage, NICD translocates to the nucleus and forms a transcriptional activation complex with RBPJ. This complex in turn recruits other coactivators and complex components, leading to regulated target gene expression. Genome-wide ChIP-seq profiling reveals both overlapping and non-overlapping chromatin-binding sites occupied by RBPJ and Notch1. Murine RBPJ-only sites are shown to be highly enriched for imputed REST (a DNA-binding transcriptional repressor) sites, whereas human RPBJ-only sites lack REST motifs and are more enriched for imputed CREB sites. Dysregulated RBPJ-dependent gene expression is seen in B-cell tumors associated with Epstein-Barr virus (EBV) and a majority of T-lymphoblastic leukemia/lymphoma (TLL) tumors having somatic Notch1 gain-of-function mutations.

Specifications

• Antigen: RBPJ
• Classification: Polyclonal
• Gene Accession No.: Q06330
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Form: Serum

• Applications: ChIP Assay, ChIP sequencing (ChIP-seq), Western Blot
• Formulation: Rabbit polyclonal in serum with 0.05% sodium azide.
• Gene Symbols: RBPJ; IGKJRB; IGKJRB1; RBPJK; RBPSUH
• Immunogen: Recombinant protein corresponding to the N-terminus of human RBPJ.
• Quantity: 100 μL
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_005340
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.

For Research Use Only