RELA (phospho T435), Rabbit anti-Human, Mouse, Rat, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA.

Supplier:  Abnova Corporation PAB25233

Catalog No. 89-126-902

Description

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM

Sequence: E-G-Tp-L-S

Specifications

• Antigen: RELA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) Immunofluorescence (1:100-1:200) The optimal working dilution should be determined by the end user.
• Gene: RELA
• Gene Symbols: RELA
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T435 of human RELA.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Liquid

• Applications: Immunofluorescence, Immunohistochemistry (PFA fixed), Western Blot
• Concentration: 1 mg/mL
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA.
• Formulation: In PBS (without Mg²⁺ and Ca²⁺), 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene Alias: MGC131774/NFKB3/p65
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5970
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

For Research Use Only