MilliporeSigma™ Rabbit anti-Ribophorin I/RPN-I, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABS1587

Catalog No. ABS1587

Description

Specifically detects Ribophorin I/RPN-I in Canine, Human, Rat samples, and it is validated for Western Blotting

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1 (EC 2.4.99.18; UniProt P04843; also known as Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67kDa subunit, Ribophorin I, RPN-I, Ribophorin-1) is encoded by the RPN1 gene (Gene ID 6184) in human. Ribophorin I is an ER transmembrane protein and a subunit of the mammalian Oligosaccharyltransferase (OST) complex. Ribophorin I binds newly synthesized target proteins and delivers them to the catalytic core of the OST complex. Association of ribophorin I with newly synthesized membrane proteins was observed after the exit of the nascent chain from the Sec61 translocon, and it was unaffected by the N-glycosylation status. Ribophorin I is reported to preferentially interact with misfolded over native ribonuclease A. In addition, Ribophorin I is shown to complex with another ER protein malectin and enhance malectin's ability to prevent the secretion of the folding-defective proteins.

Specifications

• Antigen: Ribophorin I/RPN-I
• Classification: Polyclonal
• Gene Accession No.: P04843
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Canine, Human, Rat
• Form: Serum

• Applications: Western Blot
• Formulation: Rabbit polyclonal serum with 0.05% sodium azide.
• Gene Symbols: RPN1
• Immunogen: KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Ribophorin I/RPN-I.
• Quantity: 100 μL
• Research Discipline: Signaling
• Gene ID (Entrez): NP_002941
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.

For Research Use Only