MilliporeSigma™ Rabbit anti-RLIM/Rnf12, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABE1949

Catalog No. ABE1949MI

Description

Specifically detects RLIM/Rnf12 in Hamster, Human, Mouse samples, and it is validated for ChIP, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blotting

E3 ubiquitin-protein ligase RLIM (UniProt Q9WTV7; also known as Hoxa1 regulated, LIM domain-interacting RING finger protein, R-LIM, RING finger LIM domain-binding protein, Ring finger protein 12) is encoded by the Rlim (also known as AL022832, AW743871, Ha1r, Rlim1, Rnf12) gene (Gene ID 19820) in murine species. The CLIM/LDB/NLI and RLIM/RNF12 cofactors are transcriptional coregulatory proteins characterized by their ability to bind the LIM domains of nuclear LIM proteins, including LIM homeodomain (LIM-HD) transcription factors. The association of CLIM with LIM-HDs positively regulate their biological and transcriptional activity. On the other hand, the RING finger ubiquitin ligase RLIM acts as a negative regulator of LIM-HD activity by interacting with and targeting CLIM for degradation via the ubiquitin-proteasome pathway.

Specifications

• Antigen: RLIM/Rnf12
• Classification: Polyclonal
• Gene Accession No.: Q9WTV7
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Hamster, Human, Mouse
• Form: Serum

• Applications: ChIP Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Formulation: Rabbit polyclonal serum with 0.05% sodium azide.
• Gene Symbols: Rlim; Rnf12
• Immunogen: Recombinant protein corresponding to mouse RLIM/Rnf12.
• Quantity: 100 μL
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_035406
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.

For Research Use Only