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SMAD3 (phospho S423/425), Rabbit, Polyclonal Antibody, Abnova™

Catalog No. 89115005 Shop All Abnova Corporation Products
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100 μg
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89-115-005 100 μg
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Catalog No. 89-115-005 Supplier Abnova Corporation Supplier No. PAB11304
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Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq]

Specifications

Antigen SMAD3
Applications ELISA, Immunohistochemistry (PFA fixed), Western Blot
Classification Polyclonal
Conjugate Unconjugated
Description Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.
Dilution ELISA (1:75000-1:300000) Western Blot (1:2000-1:20000) Immunohistochemistry (1:500) The optimal working dilution should be determined by the end user.
Formulation In 20mM KH2PO4, 150mM NaCl, pH 7.2 (0.01% sodium azide)
Gene SMAD3
Gene Alias DKFZp586N0721/DKFZp686J10186/HSPC193/HsT17436/JV15-2/MADH3/MGC60396
Gene Symbols SMAD3
Host Species Rabbit
Immunogen Synthetic phosphopeptide corresponding to residues surrounding S423/S425 of human SMAD3.
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 4088
Test Specificity Reactivity occurs against human Smad3 pS423 and pS425 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members.
Target Species Bovine, Chicken, Frog, Human, Mouse, Pig, Rat, Zebrafish
Content And Storage Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Form Liquid
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For Research Use Only

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