SMAD3 (phospho T179), Rabbit anti-Human, Polyclonal Antibody, Abnova™

Catalog No.	Quantity
89-121-454	100 μg

Catalog No. 89-121-454 Supplier Abnova Corporation Supplier No. PAB19947

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq]

Specifications

• Antigen: SMAD3
• Applications: ELISA, Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.
• Dilution: ELISA (1:15000-1:75000) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
• Formulation: In 20mM KH₂PO₄, 150mM NaCl, pH 7.2 (0.01% sodium azide)
• Gene: SMAD3
• Gene Alias: DKFZp586N0721/DKFZp686J10186/HSPC193/HsT17436/JV15-2/MADH3/MGC60396
• Gene Symbols: SMAD3
• Host Species: Rabbit
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding T179 of human SMAD3.
• Purification Method: Affinity purification
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4088
• Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Target Species: Human
• Content And Storage: Store at 4°C. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid

For Research Use Only