TP53 (phospho T387), Rabbit anti-Human, Polyclonal Antibody, Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of human TP53.

Supplier:  Abnova Corporation PAB29291

Catalog No. 89-130-729

Description

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]

Specifications

• Antigen: TP53
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of human TP53.
• Formulation: In PBS (without Mg²⁺ and Ca²⁺), 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene Alias: FLJ92943/LFS1/TRP53/p53
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7157
• Target Species: Human, Mouse, Rat
• Form: Liquid

• Applications: Immunohistochemistry (PFA fixed)
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (1:50∼1:100) The optimal working dilution should be determined by the end user.
• Gene: TP53
• Gene Symbols: TP53
• Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T387 of human TP53.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Test Specificity: TP53 (phospho T387) polyclonal antibody detects endogenous levels of human TP53 only when phosphorylated at threonine 387.
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

For Research Use Only