MilliporeSigma™ Rabbit anti-VE-Cadherin, Clone: Vli37,

Catalog No.	Quantity
MABT886	100 μL

Catalog No. MABT886 Supplier MilliporeSigma™ Supplier No. MABT886

Description

Rabbit Monoclonal Antibody

Specifically detects VE-Cadherin clone: Vli37 in Bovine, Mouse samples, and it is validated for Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blotting

Cadherin-5 (UniProt P55284; also known as Vascular endothelial cadherin, VE-Cad, VE-cadherin, VEcad, CD144) is encoded by the Cdh5 gene (Gene ID 12562) in murine species. Cadherins (calcium-dependent adhesion) are type-1 transmembrane proteins that form adherens junctions in tissues and play important roles in mediating cell adhesion. Cadherins are designated with a prefix that specifies their tissue association. Vascular endothelial-cadherin (VE-cadherin) is the transmembrane component of the endothelial adherens junction between vascular endothelial cells (ECs) and plays a pivotal role in endothelium integrity and in the control of vascular permeability. One characteristic of VEGF-induced vascular permeability is the phosphorylation of VE-cadherin, which leads to VE-cadherin internalization and the destabilization of adherens junctions. Likewise, VE-cadherin overexpression is shown to decrease the permeability of endothelial monolayers in vitro. VE-cadherin is initially produced with a signal peptide (a.a. 1-24) and a propeptide (a.a. 25-45) sequence, the removal of which yields tthe mature protein containing a large extracellular region (a.a. 46-599) with five cadherine repeats (a.a. 46-149, 150-256, 257-371, 372-476, 477-593), followed by a transmembrane segment (a.a. 600-620) and a cytoplasmic domain (a.a. 621-784).

Specifications

• Antigen: VE-Cadherin
• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: Vli37
• Formulation: Rabbit monoclonal IgG in supernatant with 0.05% sodium azide.
• Gene Accession No.: P55284
• Gene Symbols: Cdh5
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to the C-terminal cytoplasmic domain sequence of mouse VE-Cadherin.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_033998.2
• Target Species: Bovine, Mouse
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Form: Supernatant
• Isotype: IgG

For Research Use Only