Invitrogen™ RAD18 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA552331

Catalog No. PIPA552331

Description

Immunogen sequence: KEHGLSIQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI VQEIENIEKT RMRLEASKLN ESVMVFTKDQ TEKEIDEIHS KYRKKHKSEF QLLVDQARKG YKKIAGMSQK TVTITKEDES TE Highest antigen sequence identity to the following orthologs: Mouse - 77%, Rat - 82%.

Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.

Specifications

• Antigen: RAD18
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Rad18
• Gene Alias: 2810024C04Rik; E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; mRAD18Sc; postreplication repair protein hRAD18p; postreplication repair protein RAD18; post-replication repair protein RAD18SC; Rad18; RAD18 E3 ubiquitin protein ligase; RAD18 homolog; RAD18 homolog (S. cerevisiae); RAD18, E3 ubiquitin protein ligase; RAD18, S. cerevisiae, homolog; Rad18sc; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18; RNF73
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 56852
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.3 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9NS91
• Gene Symbols: Rad18
• Immunogen: Recombinant protein corresponding to Human RAD18. Recombinant protein control fragment (Product #RP-89467).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG