Invitrogen™ RAD18 Recombinant Rabbit Monoclonal Antibody (SN66-01)

Catalog No.	Quantity
PIMA532284	100 μL

Catalog No. PIMA532284 Supplier Invitrogen™ Supplier No. MA532284

Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.

Specifications

• Antigen: RAD18
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Western Blot
• Classification: Recombinant Monoclonal
• Clone: SN66-01
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Gene: Rad18
• Gene Accession No.: Q9NS91, Q9QXK2
• Gene Alias: 2810024C04Rik; E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; mRAD18Sc; postreplication repair protein hRAD18p; postreplication repair protein RAD18; post-replication repair protein RAD18SC; Rad18; RAD18 E3 ubiquitin protein ligase; RAD18 homolog; RAD18 homolog (S. cerevisiae); RAD18, E3 ubiquitin protein ligase; RAD18, S. cerevisiae, homolog; Rad18sc; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18; RNF73
• Gene Symbols: Rad18
• Host Species: Rabbit
• Immunogen: Synthetic peptide within Human RAD18 aa 50-99.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 362412, 56852, 58186
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG