Invitrogen™ Rat RAGE (AGER) ELISA Kit

ELISA

Catalog Number	Mfr. No.	Quantity
ERAGER	Invitrogen™ ERAGER	96 Tests
50-254-5404	Invitrogen™ ERAGERX5	5 x 96 Tests

Description

The Rat RAGE (AGER) ELISA quantitates Rt RAGE in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt RAGE. Principle of the method The Rat RAGE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.

Specifications

• Accession Number: Q63495
• Assay Sensitivity: 300 pg/mL
• Conjugate: HRP
• Protein Family: Other Proteins
• Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 81722
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Synonym: RAGE
• Test Time: 1 hrs 20 mins

• Assay Range: 0.41 to 300 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Ager
• Intraassay CV: <10
• Label or Dye: HRP
• Research Discipline: Development
• Storage Requirements: 2°C to 8°C
• Target: RAGE
• Total Assay Time: 4 hrs 45 mins