MilliporeSigma™ RalDH2 (ALDH1A2), Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABN420MI	100 μg	Unconjugated

Catalog No. ABN420MI Supplier MilliporeSigma™ Supplier No. ABN420

Description

Rabbit Polyclonal Antibody

Retinal dehydrogenase 2 (EC 1.2.1.36; UniProt Q62148; also known as Aldehyde dehydrogenase family 1 member A2, RALDH 2, RalDH2, RALDH(II), Retinaldehyde-specific dehydrogenase type 2) is encoded by the Aldh1a2 (also known as Aldh1a7, Raldh2) gene (Gene ID 19378) in murine species. It can recognize and bind both free retinal and cellular retinol-binding protein-bound retinal and oxidize it to retinoate. The enzyme is known to be active in embryos, where retinoic acid play a crucial role in early development. RALDH2-null mice do not live beyond E10.5. The three retinaldehyde dehydrogenases, RALDH1-3 or ALDH1 alpha 1-3, catalyze the irreversible oxidation of retinaldehyde to form atRA. While retinaldehyde is formed via the reversible oxidation of retinol catalyzed by retinol dehydrogenases (RDHs). The three RALDH enzymes cooperatively mediate atRA signaling during eye development through tightly regulated spatiotemporal expression patterns. In the postnatal human eye, RALDH catalytic activity can be detected in the choroid, retinal pigment epithelium (RPE), and retina. RALDH2 is most abundant in the choroid and RPE, moderate abundance in the retina, and in relatively low abundance in sclera. RALDH1 is most abundant in the choroid, in moderate abundance in the sclera, and substantially reduced in the retina and RPE. RALDH3 is most abundantly expressed during embryonic eye development, but not detectable in adult ocular fundal tissues.

Specifications

• Antigen: RalDH2 (ALDH1A2)
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: Aldh1a2, Aldh1a7, Raldh2
• Gene Accession No.: Q62148
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to an N-terminal region sequence of mouse RalDH2.
• Purification Method: Affinity Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_033048
• Test Specificity: This polyclonal antibody targets an N-terminal region sequence of human/mouse/rat RalDH2 reported by UniProt (O94788/Q62148/Q63639).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

For Research Use Only. Not for use in diagnostic procedures.