Invitrogen™ RAP1GAP Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529841

Catalog No. PIPA529841

Description

Recommended positive controls: mouse brain, rat brain. Predicted reactivity: Human (99%), Mouse (97%), Rat (98%), Zebrafish (81%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.

Specifications

• Antigen: RAP1GAP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RAP1GAP
• Gene Alias: 1300019I11Rik; 2310004O14Rik; AI427470; ARPP-90; KIAA0474; RAP1 GTPase activating protein; Rap1 GTPase-activating protein; rap1 GTPase-activating protein 1; Rap1, GTPase-activating protein 1; Rap1ga1; Rap1GAP; Rap1GAP1; RAP1GAPII; RAPGAP
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 110351, 313644, 5909
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.51 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: A2ALS5, P47736
• Gene Symbols: RAP1GAP
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Rap1GAP. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG