MP Biomedicals™ RapidScript™ Probe One-Step RT-qPCR Kit

Use the RapidScript™ Probe One-Step RT-qPCR Kit for simple, one-step RT-qPCR.

Supplier:  MP Biomedicals™ 116188000

Catalog No. MP116188000

Description

The RapidScript™ Probe One-Step RT-qPCR Kit provides the convenience of an all-in-one kit through the combination of an RT enzyme mix and a 2X qPCR mastermix to create a one-step reverse transcription and real-time qPCR amplification, all in one PCR reaction tube.

The unique formulation of the RapidScript™ RT-qPCR enzyme allows the reaction to be run either at standard conditions or fast conditions, depending on the capacity of the qPCR instrument. This allows for the RT-qPCR reaction to be completed in a total of ∼68 minutes (using the standard protocol) or ∼24 minutes (using the Fast protocol), as opposed to conventional reactions with over 50 minutes for the RT reaction and 1.5-2 hours for qPCR amplification.

• Ease of use, user flexibility and time savings when using Standard or Fast protocols (compared to conventional two-step RT and qPCR reactions)
• The mastermix is suitable for SNP genotyping assays, gene expression analysis, microarray validation and high throughput screening, among others
• ROX reference dye is provided separately, allowing for the reaction to be universally compatible with most qPCR instruments

Specifications

• Content And Storage: 2X qPCR MasterMix, 1.25 mL; RT-qPCR Enzyme Mix, 40 μL; ROX Reerence Dye, 15 μL; 10 mM Oligo(dT), 100 μL. Store at -20°C
• Polymerase: RapidScript RT-qPCR enzyme
• Passive Reference Dye: ROX
• For Use With (Application): Gene Expression

• Format: Kit
• Reaction Speed: ∼68 min. (Standard)/∼24 min. (Fast)
• Reverse Transcriptase: Yes
• No. of Reactions: 100

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.