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MP Biomedicals™ RapidScript™ Probe One-Step RT-qPCR Kit

Use the RapidScript™ Probe One-Step RT-qPCR Kit for simple, one-step RT-qPCR.

Supplier:  MP Biomedicals™ 116188000

Catalog No. MP116188000

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

The RapidScript™ Probe One-Step RT-qPCR Kit provides the convenience of an all-in-one kit through the combination of an RT enzyme mix and a 2X qPCR mastermix to create a one-step reverse transcription and real-time qPCR amplification, all in one PCR reaction tube.

The unique formulation of the RapidScript™ RT-qPCR enzyme allows the reaction to be run either at standard conditions or fast conditions, depending on the capacity of the qPCR instrument. This allows for the RT-qPCR reaction to be completed in a total of ∼68 minutes (using the standard protocol) or ∼24 minutes (using the Fast protocol), as opposed to conventional reactions with over 50 minutes for the RT reaction and 1.5-2 hours for qPCR amplification.

  • Ease of use, user flexibility and time savings when using Standard or Fast protocols (compared to conventional two-step RT and qPCR reactions)
  • The mastermix is suitable for SNP genotyping assays, gene expression analysis, microarray validation and high throughput screening, among others
  • ROX reference dye is provided separately, allowing for the reaction to be universally compatible with most qPCR instruments
Specifications

Specifications

2X qPCR MasterMix, 1.25 mL; RT-qPCR Enzyme Mix, 40 μL; ROX Reerence Dye, 15 μL; 10 mM Oligo(dT), 100 μL. Store at -20°C
RapidScript RT-qPCR enzyme
ROX
Gene Expression
Kit
∼68 min. (Standard)/∼24 min. (Fast)
Yes
100
SDS
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