RARA (phospho S77) Rabbit anti-Human, Mouse, Rat, Polyclonal , Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RARA.

Supplier:  Abnova Corporation PAB29640

Catalog No. 89-956-784

Description

Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 17468032]).[supplied by OMIM]

Specifications

• Antigen: RARA (phospho S77)
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RARA.
• Formulation: In PBS (without Mg²⁺ and Ca²⁺), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene Symbols: RARA
• Immunogen: Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S77 of human RARA.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Test Specificity: RARA (phospho S77) polyclonal antibody detects endogenous levels of human RARA only when phosphorylated at serine 77.
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.

• Applications: Immunohistochemistry (PFA fixed), Western Blot
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
• Gene Alias: NR1B1/RAR
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5914
• Target Species: Human, Mouse, Rat
• Form: Liquid