MilliporeSigma™ Rat anti-FcgRII/III Anibody, Clone: Ab93,

Catalog No.	Quantity
MABF838MI	100 μg

Catalog No. MABF838MI Supplier MilliporeSigma™ Supplier No. MABF838

Description

Rat Monoclonal Antibody

Specifically detects FcgRII/III Anibody clone: Ab93 in Mouse samples, and it is validated for Activity Assay, Flow Cytometry, Immunocytochemistry

Murine low affinity IgG Fc region receptor FcγRII and FcγRIII (UniProt P08101 and P08508, respectively) are encoded by the Fcgr2 (also known as Fcgr2b, Ly-17) and Fcgr3 gene, respectively. Four different classes of IgG Fc receptors (FcγRs) have been reported: FcγRI/CD32b, FcγRII/CD16, FcγRIII/CD16.2, and FcγRIV/CD64. FcγRII, FcγRIII, and FcγRIV (UniProt P08101, P08508, and Q8R477, respectively) are low-affinity receptors for monomeric IgG, whereas FcγRI (UniProt P26151) is the only high-affinity FcγR. FcγRs play important roles in inflammatory cell activation, clearance, presentation of antigen, and maintenance of IgG homeostasis. In addition to binding immune complex (IC), FcγRs have been shown to bind to non-IgG ligands. For example, FcγRII is shown to bind oxLDL, while FcγRIII binding to an E. coli component negatively regulates the function of macrophage receptor with collagenous structure (MARCO). FcyRII activation is also reported to transduce a negative regulatory signal against CD38 cross-linking-induced proliferation of resting mature B cells.

Specifications

• Antigen: FcgRII/III Anibody
• Applications: Functional Assay, Flow Cytometry, Immunocytochemistry
• Classification: Monoclonal
• Clone: Ab93
• Formulation: Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl without preservatives.
• Gene Accession No.: P08508; P08101
• Gene Symbols: Fcgr3; Fcgr2; Fcgr2b; Ly-17
• Host Species: Rat
• Immunogen: Whole cells expressing mouse FcgRII/III.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Inflammation and Immunology
• Primary or Secondary: Primary
• Gene ID (Entrez): CD32: NP_034317
• Target Species: Mouse
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG2a κ

For Research Use Only