CD119 Rat anti-Mouse, Brilliant Violet 421, Clone: GR20, BD Optibuild™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 740032

Catalog No. BDB740032

Description

The GR20 monoclonal antibody specifically binds to CD119, the α chain of the interferon γ(IFN- γ) receptor. This receptor is expressed by mouse leukocytes, endothelial, and epithelial cells, but not by mature erythrocytes. GR20 mAb recognizes an epitope (susceptible to certain fixatives) in the ligand-binding site of the receptor. It can block the binding of mouse IFN- γ; therefore, it can inhibit IFN- γ.mediated effects including the activation of mouse macrophages for tumor cell killing. IFN- γhas been reported to induce the expression of Ly-6A/E antigen on cell lines and normal B lymphocytes, and the GR20 antibody is capable of inhibiting the IFN- γ

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Specifications

• Antigen: CD119
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Ifngr1; IFN-γ Receptor α; IFN-gamma R alpha; Ifngr; INF-g Receptor; Cd119
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: GR20
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: BALB/c mouse monomyelocytic cell line WEHI-3
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only