CD119 Rat anti-Mouse, Brilliant Violet 711, Clone: GR20, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740706
The GR20 monoclonal antibody specifically binds to CD119, the α chain of the interferon γ(IFN- γ) receptor. This receptor is expressed by mouse leukocytes, endothelial, and epithelial cells, but not by mature erythrocytes. GR20 mAb recognizes an epitope (susceptible to certain fixatives) in the ligand-binding site of the receptor. It can block the binding of mouse IFN- γ; therefore, it can inhibit IFN- γ.mediated effects including the activation of mouse macrophages for tumor cell killing. IFN- γhas been reported to induce the expression of Ly-6A/E antigen on cell lines and normal B lymphocytes, and the GR20 antibody is capable of inhibiting the IFN- γ
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 711|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG2a, κ, also known as Brown Norway IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Ifngr1; IFN-γ Receptor α; IFN-gamma R alpha; Ifngr; INF-g Receptor; Cd119|
|BALB/c mouse monomyelocytic cell line WEHI-3|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok
For Research Use Only