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CD122 Rat anti-Mouse, Brilliant Violet 786, Clone: 5H4, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 740908

Catalog No. BDB740908

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The 5H4 antibody monoclonal antibody specifically binds to mouse CD122. CD122 is a 70-85 kDa type I transmembrane glycoprotein of the hematopoietin receptor superfamily. CD122 is also known as IL-2 Receptor beta chain (IL-2R β ) or IL-15 Receptor beta chain (IL-15R β ) as it helps form signaling receptor complexes for Interleukin-2 (IL-2) or IL-15, respectively. CD122 can combine with either CD132 (γc) alone or CD132 plus CD25 (IL-2Rα) to form inte rmediate or high-affinity IL-2 Receptor complexes, respectively. The IL-2R β chain is constitutively expressed on NK cells and at lower levels on some thymocytes, T cells, B cells, monocytes, and macrophages. IL-2R β chain expression is up regulated by IL-2. The immunogen used to generate this hybridoma was rat myel oma cells transfected with a truncated IL-2R β cDNA.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).



Brilliant Violet 786
Aqueous buffered solution containing ≤0.09% sodium azide.
IgG2a, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Il2rb; Il-2Rbeta; IL-2Rβ; IL-15Rbeta; IL-2/15 Receptor-beta; IL-2/15Rbeta
Affinity Purified
Rat myeloma cells transfected with a truncated IL-2R β cDNA
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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