CD13 Rat anti-Mouse, Brilliant Violet 605, Clone: R3-242, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740359
The R3-242 monoclonal antibody specifically binds to aminopeptidase N (APN), which has been reported to be the mouse CD13 homologue. APN is expressed in a variety of mammalian tissues, including intestinal and renal epithelial brush border, endothelium, liver, lung, and brain. In lymphoid tissues, R3-242 mAb is reported to detect APN on monocytes, macrophages, mast cells, dendritic cells, and B lymphocytes, but not on T cells, and thymocytes. However, the reported reactivity with B cells may be non-specific, via binding to the Fc γreceptors (CD16/CD32). This observation is in agreement with reports using different antibodies to mouse APN . APN may play a role in antigen presentation in the immune system. R3-242 mAb does not inhibit the enzymatic activity of APN.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
|Brilliant Violet 605|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG1, also known as Lewis IgG1|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Anpep; Alanyl aminopeptidase; Aminopeptidase N; AP-N; mAPN; Lap-1|
|Crude Aminopeptidase N Preparation from BALB/c Mouse Intestinal Epithelium|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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