CD131 Rat anti-Mouse, Brilliant Violet 421, Clone: JORO50, BD Optibuild™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 740050

Catalog No. BDB740050

Description

The JORO50 monoclonal antibody specifically recognizes the extracellular regions of the mouse CD131/ βc (Bc, AIC2B, Il3rb1, Csf2rb1) and βIL3 (BIL3, AIC2A, Il3rb2, Csf2rb2) cytokine receptor subunits. A variety of mouse cell ty pes, including multipotential hematopoietic stem cells, mast cells, megakaryocytes, eosinophils, erythroblasts, pre-B cells, and osteoclasts, express βIL-3 and βc subunits. Either βIL-3 or βc can combine with the IL-3Rα chain to form two distinct, functional (i.e., signaling), high affinity receptors for mouse IL-3. The βIL-3 subunit by itself can bind mouse IL-3 with low affinity whereas the βc subunit can not. βc (but not βIL-3) can combine with the IL-5Rα (CD125) and GM-CSFRα (CD116) subunits to form hig h affinity, signaling receptors for mouse IL-5 or GM-CSF, respectively. The immunogen used to generate the JORO50 hybridoma was the bone marrow-derived, C4-77 pro-T lymphocyte clone.

Specifications

• Antigen: CD131
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: CD131, βc, Bc, AIC2B, Il3rb1, Csf2rb1; βIL3, BIL3, AIC2A, Il3rb2, Csf2rb2
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: JORO50
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Bone Marrow-Derived C4-77 Pro-T Lymphocyte Clone
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only