CD132 Rat anti-Mouse, Brilliant Violet 421, Clone: TUGm2, BD Optibuild™

Catalog No.	Quantity
BDB740039	50 μg

Catalog No. BDB740039 Supplier BD Biosciences Supplier No. 740039

Description

Rat Monoclonal Antibody

The TUGm2 monoclonal antibody specifically binds to CD132, which is also known as, the common γsubunit ( γc). The γc chain subunit is shared by the IL-2, IL-4, IL-7, IL-9, and IL-15 receptor complexes. The γc receptor is expressed constitutively at low levels by most lymphocytes, myeloid cells, embryonic thymocytes, and lymphoid cell lines. The γc receptor is a 75-80 kD transmembrane glycoprotein which mediates signal transducing activities of cytokine receptor complexes with which it is associated. TUGm2 antibody can significantly block the binding of and proliferative response to IL-4 by CTLL-2 cells. It can also inhibit binding to high-affinity IL-2 receptors expressed by CTLL-2 cells. By itself, the TUGm2 antibody reportedly fails to significantly inhibit CTLL-2 proliferative responses to IL-2. However, when added along with TM- β 1 (Cat. No. 553359), specific for the mouse IL-2R β chain, TUGm2 was shown to synergistically reduce IL-2-driven CTLL-2 proliferative responses. TUGm2 can reduce the IL-7-induced proliferation of Con A-activated mouse splenocytes and the IL-7 responsive pre-B cell line, IxN/2b.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Specifications

• Antigen: CD132
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: TUGm2
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 421
• Description: Il2rg; IL-2Rγ; Commin gamma chain; Common γ; gamma c receptor; γc
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Rat
• Immunogen: Mouse IL-2Rγ Transfected Cell Line
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only