CD147 Rat anti-Mouse, Brilliant Violet 650, Clone: RL73 (also known as RL73.2), BD Optibuild
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740617
The RL73 monoclonal antibody specifically binds to mouse CD147. CD147 is type I membrane glycoprotein and member of the Ig superfamily. CD147 is also known as extracellular matrix metalloproteinase inducer (EMMPRIN) and gp42/basigin (BSG). The CD147 molecule is widely expressed on a variety of hemopoietic and non-hemopoietic cell types including thymocytes, lymphocytes, monocytes, granulocytes, erythroblasts and erythrocytes, endothelial cells and neoplasms. CD147 reportedly plays significant roles in the reproductive, nervous and immune systems and in tumor progression. In the immune system, CD147 can function as an adhesion molecule and signaling receptor that regulates leukocyte trafficking and immune and inflammatory responses.
The antibody was conjugated to BD Horizon BV650 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|RL73 (also known as RL73.2)|
|Brilliant Violet 650|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Bsg; Basigin; BASI; EMMPRIN; gp 42; HT7; HT-7; Neurothelin|
|Mouse EL4 Cell Subline|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
For Research Use Only