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CD153 Rat anti-Mouse, Brilliant Violet 650, Clone: RM153, BD Optibuild™

Catalog No. BDB740542 Shop All BD Biosciences Products
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BDB740542 50 μg
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Catalog No. BDB740542 Supplier BD Biosciences Supplier No. 740542
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Description

Rat Monoclonal Antibody

The RM153 monoclonal antibody specifically recognizes a 40-kDa type-II membrane glycoprotein, CD30 ligand (CD30L or CD153), which belongs to the NGF/TNF superfamily. In the presence of cytokines, CD153 stimulates B-cell proliferation, antigen-specific antibody production, and polyclonal immunoglobulin secretion. In addition, it costimulates the proliferation of activated T cells. This molecule is expressed on activated T cells, predominantly CD4+, on activated EL4 cells, and on activated Th1 and Th2 cells. The RM153 mAb inhibits the binding of CD153 to CD30.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD153
Applications Flow Cytometry
Classification Monoclonal
Clone RM153
Concentration 0.2mg/mL
Conjugate Brilliant Violet 650
Description CD30 Ligand; Cd30l; CD30LG; Tnfsf8
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Rat
Immunogen CHO cells transfected with mouse CD153 gene (Tnfsf8)
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2b κ
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