CD153 Rat anti-Mouse, Brilliant Violet 711, Clone: RM153, BD Optibuild
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740751
The RM153 monoclonal antibody specifically recognizes a 40-kDa type-II membrane glycoprotein, CD30 ligand (CD30L or CD153), which belongs to the NGF/TNF superfamily. In the presence of cytokines, CD153 stimulates B-cell proliferation, antigen-specific antibody production, and polyclonal immunoglobulin secretion. In addition, it costimulates the proliferation of activated T cells. This molecule is expressed on activated T cells, predominantly CD4+, on activated EL4 cells, and on activated Th1 and Th2 cells. The RM153 mAb inhibits the binding of CD153 to CD30.
The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy5.5 / Alexa Fluor 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 711|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|CD30 Ligand; Cd30l; CD30LG; Tnfsf8|
|CHO cells transfected with mouse CD153 gene (Tnfsf8)|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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