CD179b Rat anti-Mouse, Brilliant Violet 421, Clone: LM34, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740069
The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked CD179b ( λ 5) and CD179a (VpreB) proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The LM34 antibody reacts with λ 5 and surrogate light chain, but not VpreB alone, in transfected X63-Ag8.653 cells. It detects surrogate light chain on pro-B and pre-B cell lines, in the presence or absence of IgH, but not on IgM-positive B lymphocytes. It also detects surrogate light chain or λ 5 on the surface and in the cytoplasm of pro-B or pre-B cells from the bone marrow of normal or RAG2-deficient mice. It has been noted that the (CD45R/B220) cell-surface expression of surrogate light chain is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37°C. At the earliest stages of B lymphopoiesis, before IgH is available, the surrogate light chain associates with a complex of glycoproteins, including a nonclassical cadherin, which could be involved in selective adhesion events during B-lymphocyte development. After immunization, cell-surface λ 5 is detected on a subset of splenic Ig light chain-positive germinal-center B lymphocytes.
|Brilliant Violet 421|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG2a, κ, also known as Lewis IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|λ5; Lambda 5; VpreB1; Vpreb-2; Immunoglobulin omega chain; Ig omega|
|and V[preB], λ5, Purified soluble complex of recombinant μ IgH|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok
For Research Use Only