CD2 Rat anti-Mouse, Brilliant Violet 786, Clone: RM2-5, BD Optibuild™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 740847

Catalog No. BDB740847

Description

The RM2-5 monoclonal antibody specifically binds to the immunoglobulin superfamily adhesion molecule, CD2 (LFA-2). CD2 is a type I transmembrane glycoprotein that serves as the the major receptor for CD48 in the mouse. CD2 is involved in T-cell activation, immunoregulation, and thymocyte maturation. In the mouse, CD2 is expressed on peripheral T lymphocytes, B lymphocytes, and NK cells, and a subpopulation of intraepithelial T lymphocytes. CD2 is expressed throughout mouse thymic ontogeny, except for distinct subsets of the CD4-CD8- early thymocytes. In the mouse bone marrow, CD2 is expressed on B220+ sIg+ CD43- pre-B cells, but not on CD43+ pro-B cells. The RM2-5 antibody is one of a set of five anti-mouse CD2 monoclonal antibodies that were classified into two groups according to their mutual competition in binding to cell surface CD2 and which block CD2-mediated cell-cell adhesion.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

Specifications

• Antigen: CD2
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: LFA-2; LFA-3 receptor; Ly37; Ly-37; Lymphocyte antigen 37; T11
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2b λ

• Applications: Flow Cytometry
• Clone: RM2-5
• Conjugate: Brilliant Violet 786
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse BALB/c Thymocytes
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only