CD229.1 Rat anti-Mouse, BUV395, Clone: 30C7, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740213
The 30C7 monoclonal antibody specifically binds to CD229.1, the Ly-9.1 (Lgp-100a) surface glycoprotein alloantigen found on most thymocytes, peripheral T and B cells, bone marrow lymphoid cells, hematopoietic progenitors, and lymphomas of most strains (e.g., AKR, Balb/c, CBA, SJL). 30C7 mAb does not react with the Ly-9.2 alloantigen expressed on mice derived from the C57/J line (e.g., C57BL, C57BR, C57L, C58).
The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.
|Brilliant Ultraviolet 395|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG2a, κ, also known as Louvain, LOU/C, LOU/M IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Ly-9.1; T100; CD229; Lgp100; SLAMF3|
|SJL/J mouse splenocytes|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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