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CD229.1 Rat anti-Mouse, Brilliant Violet 650, Clone: 30C7, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 740452

Catalog No. BDB740452


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Description

Description

The 30C7 monoclonal antibody specifically binds to CD229.1, the Ly-9.1 (Lgp-100a) surface glycoprotein alloantigen found on most thymocytes, peripheral T and B cells, bone marrow lymphoid cells, hematopoietic progenitors, and lymphomas of most strains (e.g., AKR, Balb/c, CBA, SJL). 30C7 mAb does not react with the Ly-9.2 alloantigen expressed on mice derived from the C57/J line (e.g., C57BL, C57BR, C57L, C58).

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

Specifications

CD229.1
30C7
Brilliant Violet 650
Aqueous buffered solution containing ≤0.09% sodium azide.
Rat
IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
0.2mg/mL
Ly-9.1; T100; CD229; Lgp100; SLAMF3
Affinity Purified
SJL/J mouse splenocytes
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
RUO
Primary
Murine
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