CD25 Rat anti-Mouse, Brilliant Violet 510, Clone: 3C7, BD Optibuild™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 740106

Catalog No. BDB740106

Description

The 3C7 monoclo nal antibody specifically binds to CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in th e bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4, another CD25-specific antibody. The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by other CD25-specific mAbs, 7D4 and PC61. 3C7 blocks the binding of IL-2 to CD25.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: CD25
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2b κ

• Applications: Flow Cytometry
• Clone: 3C7
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: IL-2-dependent BALB/c mouse cell line
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only