CD26 Rat anti-Mouse, Brilliant Violet 786, Clone: H194-112, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 740868

Catalog No. BDB740868

Description

The H194-112 monoclonal antibody specifically binds to CD26, which is also known as, Thymocyte-activating molecule (THAM), or dipeptidyl peptidase IV (DPP IV, Dpp4). CD26 is a ~220- kDa dimer formed of identical type-II transmembrane core polypeptides which undergo variable post-translational modifications. It is a multi-functional molecule with both ectopeptidase and signal-transducing activities. Studies with specific DPP IV inhibitors suggest that the enzymatic activity is involved in the mediation of T-cell activation events. The expression of CD26 is developmentally regulated in the thymus. Resting lymphoid cells of the bone marrow and peripheral B and T lymphocytes express low levels of CD26; bone-marrow and peritoneal myeloid cells do not. CD26 is also found on epithelial cells in the kidney, liver, small intestine, and lung. Cross-linked H194-112 mAb induces proliferation of immature and mature thymocytes in the presence of either IL-1 plus IL-2 or PMA; addition of IL-2 or IL-4 to PMA further enhances the activation.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

Specifications

• Antigen: CD26
• Clone: H194-112
• Conjugate: Brilliant Violet 786
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Rat
• Isotype: IgG_2a, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Description: Cd26; Dpp4; dipeptidylpeptidase 4; THAM, DPP IV
• Format: Affinity Purified
• Immunogen: Cell-surface molecules solubilized from BALB/c mouse thymocytes
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

For Research Use Only