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CD276 Rat anti-Mouse, Brilliant Violet 711, Clone: MIH32, BD Optibuild™
Rat Monoclonal Antibody
Supplier: BD Biosciences 740829
Description
The MIH32 monoclonal antibody specifically binds to CD276, also known as B7-H3 (B7 homolog 3). CD276 is a type I transmembrane glycoprotein and member of the B7-family of regulatory proteins. The expression of B7-H3 can be induced on T cells, natural killer (NK) cells and antigen presenting cells. B7-H3 is up-regulated during the differentiation of monocytes into dendritic cells or during the interaction between dendritic cells and regulatory T cells. In addition, B7-H3 is found to be expressed on fibroblasts, fibroblast-like synoviocytes and epithelial cells. CD276 (B7-H3) can function as a positive or a negative regulator of T responses.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
CD276 | |
Monoclonal | |
0.2mg/mL | |
Cd276; B7-H3; B7 homolog 3; B7h3; B7RP-2; Costimulatory molecule | |
Rat | |
Affinity Purified | |
RUO | |
Mouse | |
IgG2a κ |
Flow Cytometry | |
MIH32 | |
Brilliant Violet 711 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Mouse B7-H3 Transfected Cell Line | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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