CD3 molecular complex Rat anti-Mouse, Brilliant Violet 510, Clone: 17A2, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 740147

Catalog No. BDB740147

Description

The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: CD3 molecular complex
• Clone: 17A2
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Rat
• Isotype: IgG_2b, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Description: CD3; CD3 epsilon; Cd3e; CD3ε; T3e
• Format: Affinity Purified
• Immunogen: γδ TCR-positive T-T hybridoma D1
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

For Research Use Only