CD31 Rat anti-Mouse, BUV395, Clone: 390, BD Optibuild™
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740239
The 390 monoclonal antibody specifically binds to CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ∽130 kDa integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is weakly expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development; this alternative splicing is involved in regulation of ligand specificity. CD38 and vitronectin receptor ( α v β 3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The 390 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.
The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.
|Brilliant Ultraviolet 395|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|IgG2a, κ, also known as Lewis IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Platelet endothelial cell adhesion molecule; Pecam1; PECAM-1; Pecam-1|
|C3H/HeJ mouse hematopoietic progenitor cell line 32D|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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