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CD4 Rat anti-Mouse, Brilliant Violet 421, Clone: RM4-5, BD Optibuild™

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BDB740007 50 μg
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Catalog No. BDB740007 Supplier BD Biosciences Supplier No. 740007
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Description

Rat Monoclonal Antibody

The RM4-5 monoclonal antibody specifically binds to the CD4 (L3T4) differentiation antigen expressed on most thymocytes, subpopulations of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells and immunosuppressive regulatory T cells), and a subset of NK-T cells. CD4 has also been reported to be detected on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. Purified RM4-5 mAb has been reported to block the binding of FITC-conjugated anti-mouse CD4 clones GK1.5 and H129.19, but not the RM4-4 clone.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD4
Applications Flow Cytometry
Classification Monoclonal
Clone RM4-5
Concentration 0.2mg/mL
Conjugate Brilliant Violet 421
Description Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4/Leu-3
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Rat
Immunogen Mouse Thymocytes (BALB/c)
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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