CD49d Rat anti-Mouse, Brilliant Violet 650, Clone: 9C10(MFR4.B), BD Optibuild
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740468
The 9C10 (MFR4.B) monoclonal antibody specifically binds to the Integrin α 4 chain (CD49d), which is expressed as a heterodimer with either of two β chains, β 1 or β 7. The α 4β 1 integrin (VLA-4, CD49d/CD29) is expressed on most peripheral lymphocytes, thymocytes, and monocytes; while the α β 7 also preferentially binds to the mucosal vascular addressin, MAdCAM-1. Although the 9C10 (MFR4.B) antibody alone has been reported to have little function-blocking activity, it can augment the inhibitory effects of mAb R1-2 (Cat. No. 553153), resulting in almost complete inhibition of VLA-4 binding to VCAM-1.
The antibody was conjugated to BD Horizon BV650 which is part of the BD Horizon Brilliant Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Integrin α4; Itga4; Integrin alpha-4; LPAM alpha; VLA-4 alpha; VLA-4a|
|Mouse (C57BL/6xA/J)F Fetal Liver Mast MC/9|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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