CD72 b and c Alloantigens Rat anti-Mouse, Brilliant Violet 421, Clone: JY/93, BD Optibuild
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 740063
The JY/93 monoclonal antibody specifically recognizes Lyb-2.2 and Lyb-2.3 (CD72 b and c alloantigens, respectively). CD72 is a 45-kDa type-II membrane protein containing a C-type lectin-like domain, an ITIM and ITIM-like sequence in the cytoplasmic tail. Recently, CD72 has been shown to negatively regulate B-cell receptor signaling. Analysis of CD72-deficient mice supports these results and shows that CD72 is involved in B-cell development. CD72-stimulated B cells show a transient association of CD19 with CD72, as well as an increase in Tyr-phosphorylation of CD19. CD72 is reported to be a ligand for CD5,5 although this is controversial. The CD72 alloantigens Lyb-2.1 (originally identified as Ly-m19.2), Lyb-2.2 (originally identified as Ly-32.2), Lyb-2.3, and Lyb-2.4 are encoded by the Cd72[a], Cd72[b], Cd72[c] , and Cd72[d] alleles, respectively. Lyb-2.2 (CD72b) is expressed on B lymphocytes, a subset of peripheral T cells, activated thymocytes, and activated T lymphocytes in A, BALB/c, CBA/H, CBA/N, C3H/He, C57BL, NZB, NZW, PL, and 129 strains. Moreover, expression of CD72b on T cells is dominant in F1 hybrid mice, and its level of expression increases upon activation. Lyb-2.3 (CD72c) is expressed on B cells of AKR, CE, SJL, and other mouse strains. Although staining of a small percentage of splenic T cells has been detected in CD72c mice, northern blot analysis has shown no expression of CD72c in T lymphocytes of these mice. JY/93 mAb does not stain cells from CD72a mice ( e.g ., CBA/J, DBA/2, SWR), and it does not stain resting thymocytes, splenic CD11b-positive cells, or NK cells. Five serological specificities of CD72 alloantigens have been described and the nomenclature CD72.1, CD72.2, CD72.3, CD72.4, and CD72.5 proposed.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
|CD72 b and c Alloantigens|
|Brilliant Violet 421|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Lyb2b and Lyb2c; Lyb-2.2 and Lyb-2.3; Lyb-2; Ly-19; Ly-32|
|BALB/c Mouse Spleen Cells|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
For Research Use Only