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CD93 (Early B Lineage) Rat anti-Mouse, BUV395, Clone: AA4.1, BD Optibuild™
Rat Monoclonal Antibody
Supplier: BD Biosciences 740275
Description
The AA4.1 monoclonal antibody specifically recognizes the Early B Lineage antigen which is also known as CD93, AA4 antigen, Ly-68, and Complement component C1q receptor (C1qRp). This 130-140-kDa type I transmembrane glycoprotein is expressed on immature B lymphocytes in the adult bone marrow and on hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, and embryonic yolk sac. Although CD93+ cells are most plentiful in adult mouse bone marrow, a smaller number of CD93+ cells which express lower CD93 levels can be detected in the adult spleen using bright fluorescent conjugates of the AA4.1 antibody or an amplified indirect immunofluorescent staining procedure. It has been observed that the staining pattern of the 493 monoclonal antibody is similar to that of the AA4.1 antibody, that both antibodies precipitate molecules of the same molecular weight, and that staining with the AA4.1 antibody is not blocked by the 493 antibody. These results suggest that the antibodies recognize separate epitopes on the same Early B Lineage antigen.
The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.
Specifications
CD93 (Early B Lineage) | |
Monoclonal | |
0.2mg/mL | |
Aa4; Cd93; C1qRp; C1qr1; Ly-68; Ly68; C1q/MBL/SPA receptor | |
Rat | |
Affinity Purified | |
RUO | |
Mouse | |
IgG2b κ |
Flow Cytometry | |
AA4.1 | |
Brilliant Ultraviolet 395 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
derived from (C57BL/6 x DBA/2)F1 mouse, Pre-B lymphoma 70Z/3 | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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