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CD93 (Early B Lineage) Rat anti-Mouse, Brilliant Violet 510, Clone: AA4.1, BD Optibuild™

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BDB740152 50 μg
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Catalog No. BDB740152 Supplier BD Biosciences Supplier No. 740152
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Description

Rat Monoclonal Antibody

The AA4.1 monoclonal antibody specifically recognizes the Early B Lineage antigen which is also known as CD93, AA4 antigen, Ly-68, and Complement component C1q receptor (C1qRp). This 130-140-kDa type I transmembrane glycoprotein is expressed on immature B lymphocytes in the adult bone marrow and on hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, and embryonic yolk sac. Although CD93+ cells are most plentiful in adult mouse bone marrow, a smaller number of CD93+ cells which express lower CD93 levels can be detected in the adult spleen using bright fluorescent conjugates of the AA4.1 antibody or an amplified indirect immunofluorescent staining procedure. It has been observed that the staining pattern of the 493 monoclonal antibody is similar to that of the AA4.1 antibody, that both antibodies precipitate molecules of the same molecular weight, and that staining with the AA4.1 antibody is not blocked by the 493 antibody. These results suggest that the antibodies recognize separate epitopes on the same Early B Lineage antigen.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD93 (Early B Lineage)
Applications Flow Cytometry
Classification Monoclonal
Clone AA4.1
Concentration 0.2mg/mL
Conjugate Brilliant Violet 510
Description Aa4; Cd93; C1qRp; C1qr1; Ly-68; Ly68; C1q/MBL/SPA receptor
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Rat
Immunogen derived from (C57BL/6 x DBA/2)F1 mouse, Pre-B lymphoma 70Z/3
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2b κ
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