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IgG1 Rat anti-Mouse, Brilliant Violet 650, Clone: A85-1, BD Optibuild™

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BDB740478 50 μg
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Catalog No. BDB740478 Supplier BD Biosciences Supplier No. 740478
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Description

Rat Monoclonal Antibody

The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

TRUSTED_SUSTAINABILITY

Specifications

Antigen IgG1
Applications Flow Cytometry
Classification Monoclonal
Clone A85-1
Concentration 0.2mg/mL
Conjugate Brilliant Violet 650
Description Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Rat
Immunogen Pooled IgG1
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only

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